Supporting Information The hinge region strengthens the nonspecific interaction between lac-repressor and DNA: a computer simulation study
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چکیده
The hinge region strengthens the nonspecific interaction between lac-repressor and DNA: a computer simulation study Lili Sun, Marcin Tabaka, Sen Hou, Lin Li, Krzysztof Burdzy, Aleksei Aksimentiev, Christopher Maffeo, Xuzhu Zhang and Robert Holyst Institute of Physical Chemistry PAS, Kasprzaka 44/52, 01-224 Warsaw, Poland Computational Biophysics and Bioinformatics, Department of Physics,Clemson University, Clemson, SC 29634, USA Department of Mathematics, University of Washington, Seattle, WA 98195-4350, USA 4 Department of Physics, University of Illinois, Urbana, Illinois 61801, United States To whom correspondence should be addressed: Institute of Physical Chemistry PAS, Kasprzaka 44/52, 01-224 Warsaw, Poland Corresponding author: E-mail: [email protected]
منابع مشابه
The Hinge Region Strengthens the Nonspecific Interaction between Lac-Repressor and DNA: A Computer Simulation Study
LacI is commonly used as a model to study the protein-DNA interaction and gene regulation. The headpiece of the lac-repressor (LacI) protein is an ideal system for investigation of nonspecific binding of the whole LacI protein to DNA. The hinge region of the headpiece has been known to play a key role in the specific binding of LacI to DNA, whereas its role in nonspecific binding process has no...
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The hinge-region of the lac repressor plays an important role in the models for induction and DNA looping in the lac operon. When lac repressor is bound to a tight-binding symmetric operator, this region forms an alpha-helix that induces bending of the operator. The presence of the hinge-helices is questioned by previous data that suggest that the repressor does not bend the wild-type operator....
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We have investigated the effects of mixed Na+: Mg2+ ionic solutions on the stability of the nonspecific lac repressor-DNA complex. The effects of Mg2+ are simply interpreted in terms of its role as a competitor (with repressor) for DNA sites. From these studies, the binding constant of the Mg-DNA complex can be determined as a function of the concentration of Na+. We have used this information ...
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The combined structural and biochemical studies on Lac repressor bound to operator DNA have demonstrated the central role of the hinge helices in operator bending and the induction mechanism. We have constructed a covalently linked dimeric Lac-headpiece that binds DNA with four orders of magnitude higher affinity as compared with the monomeric form. This enabled a detailed biochemical and struc...
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